32mm Diameter Circular Oblique Illumination COL Microscope Filter Set

Microscope Image Enhancement

360 Degree Oblique Illumination or Circular Oblique Illumination  - is a variation of bright field  microscopy  and is a powerful tool for improved contrast and enhancing detail. As a focus of research, COL and have been extensively studied and written about by many authors over the years.

 The interest in COL stems from a desire to reproduce DIC-like images at a lower cost. It is well known that COL can enhance contrast, and therefore the ability to highlight structures not easily visible under brightfield.

 The advantages of COL, are that the enhancement of resolution non directional, with very  little impact on light intensity. This is because the COL setup requires that the condenser and field iris remain fully open for better resolution enhancement. An other  advantage of oblique illumination is directional contrast which results in subjects taking on a 3D appearance.

The use of polarized light in combination with COL termed “Polar Annular Lighting” has been studied  with excellent excellent results.

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