Publisher Description

A comprehensive collection of perspectives by experts in mycobacterial molecular biology Mycobacterium tuberculosis causes one in four avoidable deaths in the developing world and kills more adults than malaria, AIDS, and all tropical diseases combined. Tuberculosis was named a global health emergency by the World Health Organization, a distinction no other disease has received. Although the study of mycobacterial genetics has expanded dramatically, with new investigations into mycobacterial growth, replication, metabolism, physiology, drug susceptibility, and virulence, most of the problems in tuberculosis control that existed in 2000 remain today. Advances in our understanding of mycobacterial genetics have been reflected in exciting recent developments. New diagnostic approaches can identify drug resistance within a few hours, promising new drugs are progressing through the pipeline and into the clinic, and a range of newly developed vaccines are being evaluated. It is an exciting time as the fruits of 30 years of intensive genetic investigation are finally beginning to emerge. Written by leading experts in the field, Molecular Genetics of Mycobacteria, Second Edition,

• Discusses key areas of current research in mycobacterial genetics
• Explains the genetics of the physiology, metabolism, and drug sensitivities of M. tuberculosis
• Presents genetic approaches for manipulating M. tuberculosis

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Molecular Genetics of Mycobacteria Second Edition A comprehensive collection of perspectives by experts in mycobacterial molecular biology Mycobacterium tuberculosis causes one in four avoidable deaths in the developing world and kills more adults than malaria, AIDS, and all tropical diseases combined. Tuberculosis was named a global health emergency by the World Health Organization, a distinction no other disease has received. Although the study of mycobacterial genetics has expanded dramatically, with new investigations into mycobacterial growth, replication, metabolism, physiology, drug susceptibility, and virulence, most of the problems in tuberculosis control that existed in 2000 remain today. Advances in our understanding of mycobacterial genetics have been reflected in exciting recent developments. New diagnostic approaches can identify drug resistance within a few hours, promising new drugs are progressing through the pipeline and into the clinic, and a range of newly developed vaccines are being evaluated. It is an exciting time as the fruits of 30 years of intensive genetic investigation are finally beginning to emerge. Written by leading experts in the field, Molecular Genetics of Mycobacteria, Second Edition, Discusses key areas of current research in mycobacterial genetics Explains the genetics of the physiology, metabolism, and drug sensitivities of M. tuberculosis Presents genetic approaches for manipulating M. tuberculosis This book is an invaluable resource for anyone interested in the molecular genetics and molecular biology of mycobacteria.

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Molecular Genetics of Mycobacteria Second Edition A comprehensive collection of perspectives by experts in mycobacterial molecular biology Mycobacterium tuberculosis causes one in four avoidable deaths in the developing world and kills more adults than malaria, AIDS, and all tropical diseases combined. Tuberculosis was named a global health emergency by the World Health Organization, a distinction no other disease has received. Although the study of mycobacterial genetics has expanded dramatically, with new investigations into mycobacterial growth, replication, metabolism, physiology, drug susceptibility, and virulence, most of the problems in tuberculosis control that existed in 2000 remain today. Advances in our understanding of mycobacterial genetics have been reflected in exciting recent developments. New diagnostic approaches can identify drug resistance within a few hours, promising new drugs are progressing through the pipeline and into the clinic, and a range of newly developed vaccines are being evaluated. It is an exciting time as the fruits of 30 years of intensive genetic investigation are finally beginning to emerge. Written by leading experts in the field, Molecular Genetics of Mycobacteria, Second Edition, Discusses key areas of current research in mycobacterial genetics Explains the genetics of the physiology, metabolism, and drug sensitivities of M. tuberculosis Presents genetic approaches for manipulating M. tuberculosis This book is an invaluable resource for anyone interested in the molecular genetics and molecular biology of mycobacteria.

Table of Contents

Contributors ix Preface xvii I. Genomes, Genomics, and Genetic Exchange 1. Gene Transfer in Mycobacterium tuberculosis: Shuttle Phasmids to Enlightenment 3
William R. Jacobs, Jr. 2. Mycobacterial Pathogenomics and Evolution 27
Daria Bottai, Timothy P. Stinear, Philip Supply, and Roland Brosch 3. BCG Vaccines 49
Vanessa Tran, Jun Liu, and Marcel A. Behr 4. Distributive Conjugal Transfer: New Insights into Horizontal Gene Transfer and Genetic Exchange in Mycobacteria 61
Keith M. Derbyshire and Todd A. Gray 5. Molecular Genetics of Mycobacteriophages 81
Graham F. Hatfull 6. Genetics of Phage Lysis 121
Madalena Pimentel Ii. Gene Expression and Regulation 7. Sigma Factors: Key Molecules in Mycobacterium tuberculosis Physiology and Virulence 137
Riccardo Manganelli 8. Transcription Factor Binding Site Mapping Using ChIP-Seq 161
Suma Jaini, Anna Lyubetskaya, Antonio Gomes, Matthew Peterson, Sang Tae Park, Sahadevan Raman, Gary Schoolnik, and James Galagan 9. Noncoding RNA in Mycobacteria 183
Kristine B. Arnvig, Teresa Cortes, and Douglas B. Young 10. Two-Component Regulatory Systems of Mycobacteria 209
Tanya Parish 11. Regulated Expression Systems for Mycobacteria and Their Applications 225
Dirk Schnappinger and Sabine Ehrt Iii. The Mycobacterial Proteome 12. Mycobacterium tuberculosis in the Proteomics Era 241
Martin Gengenbacher, Jeppe Mouritsen, Olga T. Schubert, Ruedi Aebersold, and Stefan H. E. Kaufmann 13. Structural Annotation of the Mycobacterium tuberculosis Proteome 261
Nagasuma Chandra, Sankaran Sandhya, and Praveen Anand 14. Cyclic AMP Signaling in Mycobacteria 281
Gwendowlyn S. Knapp and Kathleen A. McDonough IV. Metabolism 15. The Physiology and Genetics of Oxidative Stress in Mycobacteria 299
Bridgette M. Cumming, Dirk Lamprecht, Ryan M. Wells, Vikram Saini, James H. Mazorodze, and Adrie J. C. Steyn 16. Metabolomics of Central Carbon Metabolism in Mycobacterium tuberculosis 323
Anthony D. Baughn and Kyu Y. Rhee 17. Mycobacterial Lipidomics 341
Emilie Layre, Reem Al-Mubarak, John T. Belisle, and D. Branch Moody 18. Genetics of Mycobacterial Trehalose Metabolism 361
Rainer Kalscheuer and Hendrik Koliwer-Brandl 19. Metallobiology of Tuberculosis 377
G. Marcela Rodriguez and Olivier Neyrolles 20. Energetics of Respiration and Oxidative Phosphorylation in Mycobacteria 389
Gregory M. Cook, Kiel Hards, Catherine Vilcheze, Travis Hartman, and Michael Berney V. Genetics of Drug Resistance 21. Molecular Basis of Drug Resistance in Mycobacterium tuberculosis 413
Keira A. Cohen, William R. Bishai, and Alexander S. Pym 22. Resistance to Isoniazid and Ethionamide in Mycobacterium tuberculosis: Genes, Mutations, and Causalities 431
Catherine Vilcheze and William R. Jacobs, Jr. 23. The Molecular Genetics of Fluoroquinolone Resistance in Mycobacterium tuberculosis 455
Claudine Mayer and Howard Takiff 24. Mechanisms of Pyrazinamide Action and Resistance 479
Ying Zhang, Wanliang Shi, Wenhong Zhang, and Denis Mitchison 25. Genetic Strategies for Identifying New Drug Targets 493
Andrej Trauner, Christopher M. Sassetti, and Eric J. Rubin VI. Genetics of Membrane and Cell Wall Biosynthesis 26. Genetics of Peptidoglycan Biosynthesis 513
Martin S. Pavelka, Jr., Sebabrata Mahapatra, and Dean C. Crick 27. Genetics of Mycobacterial Arabinogalactan and Lipoarabinomannan Assembly 535
Monika Jankute, Shipra Grover, Helen L. Birch, and Gurdyal S. Besra 28. Genetics of Capsular Polysaccharides and Cell Envelope (Glyco)lipids 559
Mamadou Daffe, Dean C. Crick, and Mary Jackson 29. The Molecular Genetics of Mycolic Acid Biosynthesis 611
Jakub Paweczyk and Laurent Kremer VII. Genetics of Macromolecular Biosynthesis 30. Nucleotide Metabolism and DNA Replication 635
Digby F. Warner, Joanna C. Evans, and Valerie Mizrahi 31. Double-Strand DNA Break Repair in Mycobacteria 657
Michael S. Glickman 32. The Pup-Proteasome System of Mycobacteria 667
Nadine J. Bode and K. Heran Darwin 33. Mycobacterium tuberculosis Serine/Threonine Protein Kinases 681
Sladjana Prisic and Robert N. Husson VIII. The Mycobacterial Lifestyle, Persistence, and Macrophage Survival 34. The Spectrum of Drug Susceptibility in Mycobacteria 711
Bree B. Aldridge, Iris Keren, and Sarah M. Fortune 35. The Sculpting of the Mycobacterium tuberculosis Genome by Host Cell–Derived Pressures 727
David G. Russell, Wonsik Lee, Shumin Tan, Neelima Sukumar, Maria Podinovskaia, Ruth J. Fahey, and Brian C. VanderVen 36. Evasion of Innate and Adaptive Immunity by Mycobacterium tuberculosis 747
Michael F. Goldberg, Neeraj K. Saini, and Steven A. Porcelli 37. Mycobacterial Biofilms 773
Jacobs P. Richards and Anil K. Ojha Index 785

Review

"Molecular Genetics of Mycobacteria is an extraordinary book. It includes chapters from virtually every major scientific contributor to the genetic understanding of mycobacteria. For anyone entering the field or seeking to learn about fundamental genetic and molecular aspects of mycobacteria, this book is a necessity." Barry R. Bloom, Harvard University Distinguished Service Professor and Former Dean, Harvard School of Public Health

Review Quote

Molecular Genetics of Mycobacteria is an extraordinary book. It includes chapters from virtually every major scientific contributor to the genetic understanding of mycobacteria. For anyone entering the field or seeking to learn about fundamental genetic and molecular aspects of mycobacteria, this book is a necessity.Barry R. Bloom, Harvard University Distinguished Service Professor and Former Dean, Harvard School of Public Health

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