Preface... Table of Contents... Contributing Authors... Part I Basic Methods of Lipid Isolation and Analysis 1 Lipid Isolation from Plants Jesse D. Bengtsson, James G. Wallis, and John Browse 2. Three Methods to Extract Membrane Glycerolipids: Comparing Sensitivity to Lipase Degradation and Yield Samira Mahboub, Zachery D. Shomo, R. Maxwell Regester, Mahaa Albusharif, and Rebecca L. Roston 3. Thin-Layer Chromatography Georg Hölzl and Peter Dörmann 4. Lipid Analysis by Gas Chromatography and Gas Chromatography Mass Spectrometry Mathias Brands, Philipp Gutbrod, and Peter Dörmann 5. 14C-Tracing of Lipid Metabolism Hari Kiran Kotapati and Philip D. Bates 6. Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and Euglenophytes Masako Iwai, Shiori Shibata, Hiroyuki Ohta, and Koichiro Awai Part II Mass Spectrometry and NMR Analysis 7. Direct Infusion Mass Spectrometry for Complex Lipid Analysis Katharina Gutbrod, Helga Peisker, and Peter Dörmann 8. Fatty Acid Composition by Total-Acyl-Lipid--Collision Induced Dissociation--Time-of-Flight (TAL-CID-TOF) Mass Spectrometry Pamela Tamura, Carl Fruehan, David K. Johnson, Paul Hinkes, Todd D. Williams, and Ruth Welti 9. Targeted Analysis of the Plant Lipidome by UPLC-NanoESI-MS/MS Cornelia Herrfurth, Yi-Tse Liu, and Ivo Feussner 10. Mass Spectrometry-Based Profiling of Plant Sphingolipids from Typical and Aberrant Metabolism Rebecca E. Cahoon, Ariadna Gonzalez Solis, Jonathan E. Markham, and Edgar B. Cahoon 11. Analysis of Free and Esterified Sterol Content and Composition in Seeds using GC and ESI-MS/MS Richard Broughton and Frédéric Beaudoin 12. Techniques for the Measurement of Molecular Species of Acyl-CoA in Plants and Microalgae Richard P. Haslam and Tony R. Larson 13. Quantification of Acyl-Acyl Carrier Proteins for Fatty Acid Synthesis using LC-MS/MS Lauren M. Jenkins, Jeong-Won Nam, Bradley S. Evans, and Doug K. Allen 14. Structural Analysis of Glycosylglycerolipids using NMR Spectroscopy Wiebke Knaack, Georg Hölzl, and Nicolas Gisch Part III Lipid Isolation and Analysis from Plant Tissues Cell Compartments and Organelles 15. Analysis of Extracellular Cell Wall Lipids: Wax, Cutin and Suberin in Leaves, Roots, Fruits, and Seeds Johanna Baales, Viktoria V. Zeisler-Diehl, and Lukas Schreiber 16. Isolation of Lipid Droplets for Protein and Lipid Analysis Patrick J. Horn, Kent D. Chapman, and Till Ischebeck 17. Isolation of Plastoglobules for Lipid Analyses Denis Coulon and Claire Bréhélin 18. Isolation of Mitochondria for Lipid Analysis Sébastien Leterme, Morgane Michaud, and Juliette Jouhet 19. Collection and Analysis of Phloem Lipids Susanne Hoffmann-Benning Part IV Lipid Signaling, Lipid-Protein Interactions, and Imaging 20. Analyses of Inositol Phosphates and Phosphoinositides by Strong Anion Exchange (SAX)-HPLC Debabrata Laha, Marília Kamleitner, Philipp Johnen, and Gabriel Schaaf 21. Analysis of Phosphoinositides from Complex Plant Samples by Solid-Phase Adsorption Chromatography and Subsequent Quantification via Thin-Layer and Gas Chromatography Larissa Launhardt, Monique Matzner, Mareike Heilmann, and Ingo Heilmann 22. Studying Lipid-Protein Interactions Using Protein-Lipid-Overlay and Protein-Liposome-Association Assays Guido Ufer, Peter Dörmann, and Dorothea Bartels 23. Investigations of Lipid Binding to Acyl-CoA-Binding Proteins (ACBP) Using Isothermal Titration Calorimetry (ITC) Ze-Hua Guo and Mee-Len Chye 24. In Situ Localization of Plant Lipid Metabolites by Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI) Drew Sturtevant, Mina Aziz, Trevor B. Romsdahl, Chase D. Corley, and Kent D. Chapman Part V Lipid Databases 25. Plant Lipid Databases Peter Dörmann 26. Lipid Pathway Databases with a Focus on Algae Naoki Sato and Takeshi Obayashi Subject Index List...