1pcs Transwell Nested with Polycarbonate Film 6.5mm 3.0um Sterile Clear 3415
Corning Transwell Nested with Polycarbonate Film 6.5mm 3.0um Aseptic Clear 3415 Professional Clear
(1). Co-cultivation system:
Under the condition of pore size smaller than 3.0um, cells will not migrate through. Therefore, if the research does not involve cell motility and does not require cells to pass through the polycarbonate membrane, the pore size below 3.0um should be selected. Commonly used 0.4, 3.0μm. By planting cell A in the upper chamber and planting cell B in the lower chamber, the effects of substances secreted or metabolized by cell B on cell A can be studied.
Under the condition of pore size smaller than 3.0um, cells will not migrate through. Therefore, if the research does not involve cell motility and does not require cells to pass through the polycarbonate membrane, the pore size below 3.0um should be selected. Commonly used 0.4, 3.0μm. By planting cell A in the upper chamber and planting cell B in the lower chamber, the effects of substances secreted or metabolized by cell B on cell A can be studied.
(2). Chemotaxis assay
5.0, 8.0, and 12.0 μm membranes can be used. Cells in the upper chamber can pass through the polycarbonate membrane and enter the lower chamber. Counting the amount of cells entering the lower chamber can reflect the chemotaxis of the lower chamber components to the cells in the upper chamber.
①Chemotactic effect of cell B on cell A: Cell A is seeded in the upper chamber and cell B is seeded in the lower chamber, and the chemotaxis of cell A by substances secreted or metabolized by cell B can be studied.
② Chemotactic effect of chemokines on cells: cells are planted in the upper chamber, and a certain chemokine is added to the lower chamber to study the chemotactic effect of the chemokine on cells.
5.0, 8.0, and 12.0 μm membranes can be used. Cells in the upper chamber can pass through the polycarbonate membrane and enter the lower chamber. Counting the amount of cells entering the lower chamber can reflect the chemotaxis of the lower chamber components to the cells in the upper chamber.
①Chemotactic effect of cell B on cell A: Cell A is seeded in the upper chamber and cell B is seeded in the lower chamber, and the chemotaxis of cell A by substances secreted or metabolized by cell B can be studied.
② Chemotactic effect of chemokines on cells: cells are planted in the upper chamber, and a certain chemokine is added to the lower chamber to study the chemotactic effect of the chemokine on cells.
(3). Tumor cell migration assay
8.0 and 12.0 μm membranes are commonly used, and tumor cells are planted in the upper chamber. FBS or some specific chemokines are added to the lower chamber. The tumor cells will run to the lower chamber with high nutrient content. Migration capability.
8.0 and 12.0 μm membranes are commonly used, and tumor cells are planted in the upper chamber. FBS or some specific chemokines are added to the lower chamber. The tumor cells will run to the lower chamber with high nutrient content. Migration capability.
(4). Tumor cell invasion assay
8.0 and 12.0 μm membranes are commonly used, and the principle is similar to that of tumor cell migration experiments. The upper chamber is planted with tumor cells, and FBS or some specific chemokines are added to the lower chamber. The tumor cells will run to the lower chamber with high nutrient content, but different from the tumor cell migration experiment, the upper chamber of polycarbonate membrane is covered on the side. A layer of matrigel is used to imitate the extracellular matrix in vivo. If cells want to enter the lower chamber, they must first secrete matrix metalloproteinases (MMPs) to degrade the matrigel before passing through the polycarbonate membrane. Counting the amount of cells entering the lower chamber can reflect the invasive ability of tumor cells.
8.0 and 12.0 μm membranes are commonly used, and the principle is similar to that of tumor cell migration experiments. The upper chamber is planted with tumor cells, and FBS or some specific chemokines are added to the lower chamber. The tumor cells will run to the lower chamber with high nutrient content, but different from the tumor cell migration experiment, the upper chamber of polycarbonate membrane is covered on the side. A layer of matrigel is used to imitate the extracellular matrix in vivo. If cells want to enter the lower chamber, they must first secrete matrix metalloproteinases (MMPs) to degrade the matrigel before passing through the polycarbonate membrane. Counting the amount of cells entering the lower chamber can reflect the invasive ability of tumor cells.
Packing: 1pcs
Warranty period: 120 day
Warranty period: 120 day
On Jul 24, 2022 at 21:24:18 PDT, seller added the following information:
