Manufacturer: Leica Microsystems

Model: DMi6000B CS + TCS SP5 Confocal Microscope System

Laser: HeNe (543nm, 594nm, 633nm) /  Argon (458nm, 476nm, 488nm, 496nm, 514nm) / Diode UV 405nm

Status: The extensive equipped system is completely reinstalled on a brand new computer with WIN10 and Leica LAS AF273, reinitialized and tested by Leica Service Engineer with Service report and Maintenance Batch on the device. The System is fully functional tested and ready to deliver good performance.

System Description:
Confocal microscope system with a broad range of scan speeds suited for high-resolution morphological imaging, as well as multispectral imaging.

- 350-900 nm spectral detection range with 5 nm spectral resolution and sub-micron spatial resolution
- Suitable for most fluorophores from DAPI to Cy5
- Measuring up to five dimensions, including x, y, z, time and spectrum
- Capable of multi-dimensional fluorescence qualitative and quantitative measurement, FRET, FRAP, FLIP, FLAP and other photobleaching-related applications
- Suitable for imaging fixed specimen (No environmental chamber).

Internal Detectors

- Spectral detection channels: PMT1, PMT2, PMT3
- Brightfield transmission channel: PMT Trans

Multi-dimension capability: xyz, xzy, xt, xyt, xzt, xyzt, xy, xz, xyt, xyz

Stage:
- Manual moving X, Y stage
- Motorized z stage (piezo-controlled) with 166.68 µm scanning range

Zoom range: 1x-32x

Pinhole size: 20 µm-600 µm

Contrast enhancement options: Brightfield, DIC and polarized

Software: Leica LAS AF, Version 2.7.3 on Windows 10, for image acquisition and processing

Scanners

Conventional scanner: 10-1400 Hz (2-3 frames/sec at 512 x 512 pixels)

Internal detectors

Reflection/fluorescence mode (spectral detection range 400-800 nm)

- Spectral detection channels: PMT1, PMT2, PMT3
- Additional PMT in transmission mode

Type         Wavelenght (nm)                      Dyes and use
UV            405                                                DAPI, Hoechst
Argon       458, 476, 488, 496, 514              CyanFP, GFP, Alexa488, CY2, YFP
HeNe        543                                               Alexa 546, Alexa 555
HeNe        594
HeNe        633                                               Cy5, Alexa 647

- An acousto-optical tunable filter (AOTF) controls the intensity of the visible excitation laser light.
- An acousto-optical beam splitter (AOBS) is used to split excitation light and emission light instead of filter blocks.

Microscope
Leica DMI 6000B CS Trino inverted microscope with motorized XYZ-stage

Objectives:

- HCX PL FL L 40x/0.60 CORR PH2 0-2/C (
- HCX PL APO 63x/1.40-0.60 Oil. Lbd. BL
- HC PL FLUOTAR 10x/0.30 Ph1
- HCX PL APO 40x/1.25 Oil Ph3 CS
- HCX PL FL L 63x/0.70. CORR
- HI PLAN 20x/20X/0.40

Fluorescent Source
Leica Lamphouse 106z HG 100W light source with LEJ EBQ100 Mercury Burner Power Supply for fluorescence excitation.

Fluorescence Filters:

- A4
- L5
- Y5
- N2.1
- Analyzer

Condition:
The extensive equipped system is completely reinstalled on a brand new computer with WIN10 and Leica LAS AF273, reinitialized and tested by Leica Service Engineer with Service report and Maintenance Batch on the device. The System is fully functional tested and ready to deliver good performance.

Complete German Configuration Summary:

156504116            SuperZ Galvo Tisch Typ H
156504114            Einsatz rot Typ H         
156504145            3 Plattenkreuztisch mot SP5 invers
15500325              Vorschaltgerät ebq 100 iso-l
15000906000001 PMT-Modul wassergekühlt SP5/SP8
155934681            Optical Outfit DMI 6000 CS w. ICT
11506239              HI PLAN 20x/20X/0.40
15506203              HCX PL FL L 40x/0.60 CORR PH2 0-2/C
15506192              HCX PL APO 63x/1.40-0.60 Oil. Lbd. BL
15506507              HC PL FLUOTAR 10x/0.30 Ph1
15506181              HCX PL APO 40x/1.25 Oil Ph3 CS
15506216              HCX PL FL L 63x/0.70. CORR
15521505              Lichtringset f. Kondensor S23/S28
156501100            Basismodul AOBS
156504750            Bedienkonsole programmierbar mit LCDs
156501120            konventionelles Scanningsystem SP5 II
156504500            X1 Port Emission Access
156502123            Imaging Laser ROGB für AOBS
156501201            Laser Port UV/405nm
156502300            Imaging Laser 405nm cw 50mW
156502352            AOTF UV/405
156502379            UV Optik 09
156504201            BF Detektor für DMI
156503122            Workstation Plus 2x LCD
15522023              Scanningtisch
15500138              HF-Höchstdruck-Lampe 12V 100W
156501330            SP Kanal 03
156501390            Konfokal Detektor IMG
15541537              C-Mount Adapter HC 0.63X
15513874              Filtersystem A4
15513880              Filtersystem L5
15513888              Filtersystem Y5
                                Filter N2.1
                                Analyzer
155933630            DMI 6000 BP CS Trino
156504100            SuperZ Galvo Tisch invers
15504111              Fl-Lamp Holder for DMI, straight