Polyamide Membrane Chromatography Common Solvent System
Compound Category: Coated solvent
Flavonoids: chloroform: methanol (96:4); chloroform: methanol: butanone (12:2:1); chloroform: methanol: formic acid (60:38:2);
Chloroform: methanol (60:40); chloroform: methanol: pyridine (70:22:8); chloroform:methanol: phenol (64:28:8);
Benzene: methanol: methyl ethyl ketone (90: 6: 4);
Phenols: Acetone: Water (1:1); Benzene: Methanol: HAC (45:8:4); Cyclohexane: HAC (93:7); 10% HAC
Terpenoids: n-hexane: benzene: HAC (4:1:0.5); petroleum ether: benzene: 10% HAC (10:10:5)
Sugar: ethyl acetate: methanol (8:1) n-butanol: acetone: water: HAC (6:2:1:!)
Amino acid derivatives: benzene: HAC (8:2 or 9:1); 50% HAC; formic acid: water (1.5:100);
Ethyl acetate: methanol: HAC (20:1:1); 0.05 M trisodium phosphate: ethanol (3:1)
Dimethylformamide: HAC: Water: Ethanol (5:10:30:20)
Steroids, steroids, steroids: ethane: acetone (4:1); chloroform: acetone (4:1); methanol: water: formic acid (60:35:5);
Ethyl acetate: methanol: water: formic acid (50:20:25:5);
Alkaloid: cyclohexane: ethyl acetate: n-propanol: dimethylamine (30:25:0.9:0.1); water:ethanol: dimethylamine (88:12:0.1)
Flavonoids: methanol: HAC: water (90:5:5); methanol: water (4:1) ethanol: water (1:1); acetone: water (1:1);
Isopropanol: water (3:2); ethyl acetate: ethanol (6:4); chloroform: methanol (7:3); n-butanol: ethanol: water (1:4:5)