Object plate (microscope) biological object plate slide glass cover rectangular teaching instrument

 

1. Size: 76.2 × 25.4mm(3 "× 1") thickness: 1.0-1.2mm 50 pieces
2. Material
       Object plate is the glass cover used to place the experimental material during the experiment, which is rectangular, thick and has good light transmission; Cover glass is covered on the material to avoid contact between liquid and objective lens, so as not to pollute objective lens, it is square, thin and has good light transmission.
Function
       Object plate is the glass cover used to put things when observing things with a microscope, and then place the cover glass on it for observing cells.

 

III. Usage

1. Film preparation method is a production method that evenly coating materials on object plate.

Film preparation materials include single-celled organisms, small algae, blood, bacterial culture solution, loose tissues of animals and plants, sperms, anther, etc.

Note when film preparation:
(1) object plate must be cleaned.
(2) object plate should be flat.
(3) the coating shall be uniform. Apply the drop to the right in the middle of the object plate and use spreadability such as dissecting blade or toothpick.
(4) the coating should be thin. Use the other object plate as a push piece, along the drop there is essence of the object plate surface (the angle between the two object plate should be 30 °-45 °) gently pushed from right to left, painted into a uniform thin layer.
(5) fixed. If fixing is required, chemical fixing agent or drying method (bacteria) can be used for fixing.
(6) dyeing. Methylene blue is used for bacteria and Wright dye solution is used for blood. Stain solution cover all coated surfaces.
(7) Rinse. Use absorbent paper towels to suck or bake.
(8) seal. For long-term storage, use Canadian gum seal.

2. The tablet method is a production method that places biological materials between object plate and cover plate and applies certain pressure to disperse tissue cells.

3. Mounting method is the method of sealing biological materials into glass slide specimens as a whole, which can be used to make temporary or permanent mounting.

Mounting materials include: tiny organisms such as algae, sponge, Amoeba and C. Elegans; Hydra, leaf epidermis of plants; Wings, feet and mouthparts of insects, human oral epithelial cells, etc.

Attention should be paid to the production of mounting method:
(1) when holding object plate, pay attention to being flat or on the platform. When dripping water, the amount of water should be appropriate to be covered by cover glass.
(2) materials should be spread out with dissecting needles or tweezers without overlapping and flat on the same plane.
(3) when putting cover glass, slowly cover the water droplets from one side to prevent bubbles.
(4) When dyeing, place a drop of dyeing droplets on one side of cover glass and attract them from the other side with absorbent paper towels to evenly color the specimens under cover glass. After coloring, use the same method, drop a drop of water, suck out stain solution, and observe under a microscope.

4. Slices are glass slide specimens made from slices cut from organisms.

Due to different requirements, the blade can be used for freehand slicing, or the tissue block can be embedded in paraffin wax or collodion or frozen at low temperature, and sliced by slicer. Cut into 5~10 micron slices for optical microscope observation. Ultra-thin slices made by embedding tissue blocks with epoxy resin or methacrylic acid have a thickness of 20~50 nm for observation under electron microscope. The slices used for general teaching, such as root tip and stem, are generally called paraffin section.

Cleaning method
Cleaning method: wash it with clean water or alcohol, and then wipe it clean with cotton or gauze (it is recommended to use lens wiping paper, fingers should avoid touching object plate, so as not to leave fingerprints on it, which will affect the next observation and use)