The QIAfilter Plasmid Kits combines QIAfilter Cartridges for rapid clearing of bacterial lysates by filtration instead of centrifugation, with proven QIAGEN-tips containing anion-exchange resin for efficient plasmid purification. Yields of up to 10 mg (giga), 2.5 mg (mega), 500 µg (maxi), and 100 µg (midi) transfection-grade, high-copy plasmid DNA are achieved from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium).

QIAfilter Cartridges, provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour.

As an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation, you can use the QIAfilter Mega-Giga Cartridges (cat. no. 19781) with the QIAGEN Plasmid Mega Kit (cat. no. 12181) and the QIAGEN Plasmid Giga Kit (cat. no. 12191). Visit the QIAGEN plasmid kits web page to learn more.
QIAfilter Maxi and Midi Cartridges use a syringe format and lysates are cleared rapidly by pushing the liquid through the filter.

The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation. Prepacked QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.