Autore: Adeeb Shehzad
Formato: Copertina flessibile
Pagine: 144
Data Pubblicazione: 2020-06-16
Edizione: 1
Lingua: English
Descrizione:
This book underpins the Prp4 and PGE2induced cancer cell's survival and underlying mechanism of anticancer and radiosensitizing effects of curcumin (a derivative of turmeric) in colorectal carcinoma as well as in B16F10induced tumor xenograft. Curcumin and ionizing radiation (IR) inhibited HCT15 cell proliferation, induced apoptosis, and reduced tumor volume in C57BL6/J mice. Transfection of HCT15 cells with splicing factor Prp4 clone reverses curcumin and IRinduced cell death. However, knockdown of Prp4 with siRNA diminished the protective effects of Prp4 against curcumin and IRinduced apoptosis in HCT15 cells. Curcumin treatment also restored the IRinduced cell death as well as greatly reduced the tumor volume by regulating the level of antioxidant enzymes. PGE2, an inflammatory mediator, inhibited curcumininduced oxidative stress apoptosis by regulating survival pathways such as COX2 and PKA, Ras/Raf/Erk, and NF?B through EP2 receptor. Taken together, splicing factor Prp4 and inflammatory mediator PGE2 enhances the survival as well as protect the cancer cells death by activating the antioxidant enzymes, Ras/Raf/Erk, and NF?B pathways.