Quagen QIAamp Fast DNA Tissue Kit, Cat No. 51404 This is a Quagen QIAamp Fast DNA Tissue Kit, Cat No. 51404. What you will get: For 50 preps: QIAamp Spin Columns, QIAGEN Proteinase K, RNase A, Tissue Disruption Tubes, Buffers Genotyping and other DNA analyses using PCR or next-generation sequencing (NGS) require a suitable amount of high-quality genomic DNA as starting material. The most common bottleneck to obtaining high-quality DNA is tissue lysis. Fibrous or other hard-to-lyse tissues require lengthy and tedious protocols that often result in low yields of low-quality DNA. The QIAamp Fast DNA Tissue Kit, based on proven QIAGEN technologies, overcomes this bottleneck by providing innovative disruption tubes that significantly improve yield and shorten the overall time to result. The procedure can be fully automated on the QIAcube Connect. Want to try this solution for the first time? Request a trial kit quote.
This is my item N1638 N1641. 4 unopened kits were available at listing time.
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As well as speeding up the total time to extract DNA, the mechanical disruption step and thorough lysis protocol increase DNA yield. Improvements in yield from different tissue types have been evaluated using the QIAxpert and a fluorometric assay. The results show that the QIAamp Fast DNA Tissue Kit is capable of extracting up to tenfold more DNA compared to standard methods, while also providing one of the fastest protocols.
Most NGS applications require high-molecular-weight DNA (>10 kb) to allow for larger spans to be sequenced and assembled with bioinformatic tools. Some disruption methods may fragment the DNA through shearing, which can affect downstream analysis. Compared to other kits that show high fragmentation down to a few kb, the QIAamp Fast DNA Tissue Kit allows you to obtain DNA between 25 and 50 kb and is therefore very well suited to any PCR or NGS application.Principle
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