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SDA medium, Sabouraud Dextrose Agar | 250g
Sabouraud Dextrose Agar is a medium is a type of agar growth medium containing peptones. It is used to cultivate dermatophytes and other types of fungi, and can also grow filamentous bacteria such as Nocardia. It has utility for research and clinical care.
Per 1000mL of SDA medium contains
Peptone 10g
Dextrose 40g
Agar * 15g
pH 5.6
Chloramphenicol 50 mg (Optional)
Gentamicin 25 mg (Optional)
* Tissue Culture Grade | Gel strength (15g/L, 20°C) ≥1400g/cm2
Preparation
Weigh 65 g of SDA medium, dissolve in distilled or MQ water and make the final volume to 1L. Autoclave at 121°C for 10 minutes.
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