Principles of Cloning, Second Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species—including mice, sheep, cattle, and non-mammals—is considered as well.   The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine – Dr John Gurdon; the cloning of the first mammal from a somatic cell – Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual – Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell – Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas.

List of Contributors.
Preface.
Introduction.
Historical Perspective.
PART ONE-BASIC BIOLOGICAL PROCESES
Activation of Mammalian Oocytes.
The Nucleus.
Nuclear Reprogramming: Biological and Technological Constraints.
Plasticity of Somatic Nucleus by Epigenetic Reprogramming via Cell Hybridization.
Cell Cycle.
Determinants of Pluripotency in Mammals.
Cloning and Aging.
PART TWO-METHODS
Micromanipulation Techniques for Cloning.
Microinsemination and Nuclear Transfer with Male Germ Cells.
Development of Viable Mammalian Embryos in Vitro: Evolution of Sequential Media.
Genetic and Phenotypic Similarity Among Members of Mammalian Clonal Sets.
Genetic Modification and Cloning in Mammals.
Pregnancy and Neonatal Care of Clone Animals.
Donor Cell Type and Cloning Efficiency in Mammals.
PART THREE-CLONING BY SPECIES
The Cloning of Amphibians.
Cloning of Fish.
Cloning of Mice.
Cloning of Rabbits.
Nuclear Transfer in Swine.
Cloning of Cattle.
Cloning of Sheep.
Cloning of Goats.
PART FOUR-CURRENTLY SOUGHT AFTER SPECIES
Cloning of Endangered Species.
Cloning of Rats.
PART FIVE-NUCLEAR TRANSFER IN PRIMATES
Cloning in Non-Human Primates.
PART SIX-APPLICATIONS
Nuclear Transfer for Stem Cells (CRNT).
Current Research and Commercial Applications of Cloning Technology.
Transgenic Cloned Goats and the Production of Therapeutic Proteins.
PART SEVEN-ETHICAL AND LEGAL AFFAIRS
Ethical Implications of Cloning.
FINAL REMARKS
Mammalian Cloning- Challenges for the Future.

Subject Areas: Animal breeding [TVHB], DNA & Genome [PSAK1], Genetics [non-medical PSAK]