Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists.

1. History of plant tissue culture2. Setup of a tissue culture laboratory3. Media components and preparation4. Explant preparation5. Contamination6. Callus induction7. Regeneration and morphogenesis8. Woody shrubs and trees9. Haploid plants from another culture10. Embryo rescue11. Meristem culture for virus-free plants12. In Vitro propagation for commercial production of ornamentals13. Protoplast isolation and fusion14. Agrobacterium-mediated transformation of plants15. Genome edition and RNAi

Subject Areas: Botany & plant sciences [PST], Cellular biology [cytology PSF]