Phenotyping of Human iPSC-derived Neurons
Patient-Driven Research
Examines the steps in a preclinical pipeline that utilizes iPSC-derived neuronal technology and what considerations and best practices should be implemented
Elizabeth D. Buttermore (Edited by)
9780128222775
Paperback / softback, published 15 September 2022
372 pages
23.5 x 19 x 2.4 cm, 0.75 kg
Phenotyping of Human iPSC-derived Neurons: Patient-Driven Research examines the steps in a preclinical pipeline that utilizes iPSC-derived neuronal technology to better understand neurological disorders and identify novel therapeutics, also providing considerations and best practices. By presenting example projects that identify phenotypes and mechanisms relevant to autism spectrum disorder and epilepsy, this book allows readers to understand what considerations are important to assess at the start of project design. Sections address reproducibility issues and advances in technology at each stage of the pipeline and provide suggestions for improvement. From patient sample collection and proper controls to neuronal differentiation, phenotyping, screening, and considerations for moving to the clinic, these detailed descriptions of each stage of the pipeline will help everyone, regardless of stage in the pipeline. In recent years, drug discovery in the neurosciences has struggled to identify novel therapeutics for patients with varying indications, including epilepsy, chronic pain, and psychosis. Current treatment options for such patients are decades old and offer little relief with many side effects. One explanation for this lull in novel therapeutics is a lack of novel target identification for neurological disorders (and target identification requires exemplar preclinical data). To improve on the preclinical work that often relies on rodent modeling, the field has begun utilizing patient-derived induced pluripotent stem cells (iPSCs) to differentiate neurons in vitro for preclinical characterization of neurological disease and target identification.
Section I: Best practices and considerations when designing a new project 1. iPSC culture: best practices from sample procurement to reprogramming and differentiation 2. Phenotypic assay development with iPSC-derived neurons: technical 3. Derivation of cortical interneurons from human pluripotent stem cells to model neurodevelopmental disorders 4. Development of transcription factor-based strategies for neuronal differentiation from pluripotent stem cells 5. Differentiation of Purkinje cells from pluripotent stem cells for disease phenotyping in vitro 6. Brain organoids: models of cell type diversity, connectivity, and disease phenotypes Section II: The use of iPSC-derived neurons to study neurological disorders 7. Human models as new tools for drug development and precision medicine 8. Use of cerebral organoids to model environmental and gene x environment interactions in the developing fetus and neurodegenerative disorders 9. iPSC-derived models of autism: Tools for patient phenotyping and assay-based drug discovery 10. Probing the electrophysiological properties of patient-derived neurons across neurodevelopmental disorders 11. Advantages and limitations of hiPSC-derived neurons for the study of neurodegeneration Section III: New technology, industry perspective, and transitioning to the clinic 12. Developing clinically translatable screens using iPSC-derived neural cells 13. Gene editing hPSCs for modeling neurological disorders 14. Cell therapy and biomanufacturing using hiPSC-derived neurons 15. Ethical considerations for the use of stem cell-derived therapies
Subject Areas: Neurosciences [PSAN]