AmScope  40-600X Phase Contrast Fluorescence Inverted Microscope Model IN480T-FL

Key Features:

  • Designed for fluorescence microscopy, this microscope uses a high-power arc-lamp to cover a wide excitation range.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • Five plan-achromatic objective lenses provide magnifications ranging from 40X to 600X
  • The infinity-corrected optical system eliminates certain optical errors when using filters and other in-line components, ensuring highly-precise images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • Brand Name: AmScope     Model No: IN480T-FL

    The IN480T-FL provides high-resolution optics and a versatile dual-illumination system with Koehler transmitted lighting for brightfield and phase-contrast, and wide-spectrum episcopic lighting for fluorescence. The stable and ergonomic inverted form accommodates larger specimen containers, with multiple specimen templates and mechanical stage controls to improve workflow.

    The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging.

    The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly-precise images. Five brightfield objective lenses provide a wide magnification range from 40X to 600X, with plan-achromatic corrections for high resolution, accurate colors, and edge-to-edge sharpness. Additionally, three phase-ring equipped objective lenses can be used for phase-contrast observation.

    A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

    The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

    This microscope operates on 90-240V wide-band voltage for use in multiple international regions.

    Fluorescence

    Filters

    Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth Common Stains
    B1 480nm/30nm 505nm 535nm/40nm Alexa Fluor 488, Cy2, FITC
    G1 560nm/40nm 600nm 635nm/60nm Texas Red, Cy3.5, Mito Tracker Red
    Mercury HBO Excitation

    Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

    Band Peak Wavelength
    UV 334nm, 365nm
    V 405nm
    B 436nm
    G 546nm
    Y 578nm


    Specifications

    Optical System Infinity-corrected
    Head Trinocular, 45° incline
    Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-76mm
    Ocular Diameter 30mm
    Eyepieces 10X FN22mm plan wide-field
    Photo-port CX-type height-adjustable, 23mm tube
    Objective Turret quintuple
    Focusing System
    Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
    Fine Division0.002mm
    Coarse Stroke38mm
    Fine Stroke0.2mm
    Stage fixed-height, mechanical X-Y translation
    Stage Dimensions 160mm x 250mm
    stage extension: 70mm x 180mm
    Stage X-Y Travel Range 120mm x 80mm
    Specimen Holders Terasaki plate, Petri dish
    Transmitted Illumination 6V 30W halogen
    variable intensity
    centerable lamp
    Condenser NA 0.3, centerable
    Condenser Working Distance 72mm maximum
    Phase Contrast Annuli 4X/10X and 20X/40X
    Power 90-240VAC 50/60Hz wide-band

    Objective Lenses

    Magnification Corrections NA Working Distance Cover-glass Thickness
    4X plan achromatic 0.13 10.4mm 1.5mm
    10X plan achromatic 0.25 7.30mm 1.5mm
    20X plan achromatic 0.40 6.79mm 1.5mm
    40X plan achromatic 0.60 3.08mm 1.5mm
    60X plan achromatic 0.70 1.71mm 1.5mm
    10X phase-ring 0.25 7.30mm 1.5mm
    20X phase-ring 0.40 6.80mm 1.5mm
    40X phase-ring 0.60 3.08mm 1.5mm

    Magnification Chart

    Eyepiece Objective
    4X 10X 20X 40X 60X
    10X 40X 100X 200X 400X 600X


    Contents

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair of 10X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
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    Shipping & Handling:

  • FREE Shipping to Contiguous USA (the lower 48 States)
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    Return Policy

    We offer 30 days from the date of receipt (verified by tracking information) to request a return on a unit. To do so, please send us a message through eBay member to member messages, with your reason for return, requesting an RMA number.

    Note that shipping costs are borne by the customer. Returns requested after 30 days are not valid, and any returns sent without an RMA number will be returned to sender at the sender's expense. Also, please insure all items shipped back, as damage to items by the courier during the return shipment to us are not covered otherwise.

    Please keep all original packing materials, as they are required for return (or a 10% restocking fee will be charged). Returned items must be in new/usable condition unless defective on arrival to you.

    Warranty Information

    We, AmScope, provide all warranty services. Please feel free to contact us whenever you need help by messaging us from the store you purchased the product from.

    Note: Buyers bear the cost of shipping and handling cost round trip for warranty services. Warranty does not cover consumable accessories and parts such as bulbs.

    Warranty service times vary throughout the year, and are processed in a first come, first serve manner, when parts are available and in stock. Most repairs/replacements are done within 1-2 weeks, however if there is a long line during the busy season, or if your parts need to be ordered from our manufacturer, processing times can be up to 6-8 weeks. We appreciate your patience and understand everyone has a critical need for their unit, and do everything we can to ensure that these requests are handled as quickly as possible.

    Defective/Missing Items

    For defective items or any missing items from your order, please contact us right away to let us know of the issue.

    We allow a 30 day period (from the date of receipt of the item in the mail) to report such issues--any items missing after that time frame will need to be purchased, and any defective items will be treated as warranty issues for repair or replacement only (no returns for refund).

    For missing items and broken items, we do require a picture of the shipping label on the box, and of the items that arrived with your unit for verification before we can send any missing items or replacements to you.

    Return Procedure

    To initiate an RMA, please send us a message through the store you purchased it from. Include your order number, reason for return, and if any pictures are required for your claim, please include them also.

    From there, we will process within 24-72 hours, and if approved, issue you an RMA number and instructions on where to mail your unit back to. If qualifying for return shipping covered by us (for defective items within 30 days of receipt), your return label may take another 24-48 hours to process and be received in your email inbox.

    Follow the issued instructions, and once received, we will process the item in, and check to ensure everything is there. We will contact you when the item is ready for return shipping (if a warranty request), or simply issue your refund with the appropriate deductions (if any) once processed.

    Processing is done first come, first serve, and can take between 1-4 weeks to process (depending on how many requests we currently have in queue). We thank you for your patience while we work on your request.