In the American Civil War, the regiment was made up of a majority of abolitionists from the Brooklyn area. It was led first by Colonel Alfred Wood. The 14th Brooklyn was involved in heavy fighting, including most major engagements of the Eastern Theater. Their engagements included the First and Second Battles of Bull Run, the Battles of Antietam, Fredericksburg, Chancellorsville, Gettysburg, The Wilderness, and Spotsylvania Court House. During the war, the men of the 14th Brooklyn were well known by both armies and throughout the country for their hard drill, hard fighting, and constant refusal to stand down from a fight. During their three years of service they never withdrew from battle in unorderly fashion.